Insights into the genetic organization of the Bacillus mycoides cryptic plasmids pDx14.2 and pSin9.7 deduced from their complete nucleotide sequence

Carmen Di Franco; Tiziana Santini; Giuseppe Pisaneschi; Elena Beccari

doi:10.1016/j.plasmid.2005.05.002

Insights into the genetic organization of the Bacillus mycoides cryptic plasmids pDx14.2 and pSin9.7 deduced from their complete nucleotide sequence

Plasmid. 2005 Nov;54(3):288-93. doi: 10.1016/j.plasmid.2005.05.002. Epub 2005 Jul 22.

Authors

Carmen Di Franco¹, Tiziana Santini, Giuseppe Pisaneschi, Elena Beccari

Affiliation

¹ Dipartimento di Genetica e Biologia Molecolare.

PMID: 16040120
DOI: 10.1016/j.plasmid.2005.05.002

Abstract

Bacillus mycoides, a member of the Bacillus cereus group of bacteria, can be easily distinguished from close species because of colony shape, made by filaments of cells, resembling fungal hyphae, curving clock- or counterclockwise depending on the strain. Two plasmids, one from a strain curving to the right (pDx14.2), the other from a strain curving to the left (pSin9.7), were sequenced and analyzed for gene content and replication mode. Rolling-circle replication modules and mobilization proteins were found, very similar to those of other plasmids of the B. cereus group bacilli, mostly Bacillus thuringiensis living in the same ecosystem, suggesting active plasmid exchange in nature.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Bacillus / genetics*
Base Sequence
Molecular Sequence Data
Open Reading Frames
Plasmids / chemistry
Plasmids / genetics*
Sequence Homology, Amino Acid