Identification of unique bacterial gene segments from Streptococcus mutans with potential relevance to dental caries by subtraction DNA hybridization

J Clin Microbiol. 2005 Jul;43(7):3508-11. doi: 10.1128/JCM.43.7.3508-3511.2005.

Abstract

Using DNA subtractive hybridization, 49 unique gene segments were identified from a strain of Streptococcus mutans that was isolated from a patient with severe early childhood caries (S-ECC). Further hybridization with DNA from other S. mutans strains isolated from both caries-active and caries-free subjects yielded five unique sequences of DNA common to strains associated with S-ECC.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Child
  • Child, Preschool
  • DNA, Bacterial / analysis*
  • DNA, Bacterial / genetics
  • Dental Caries / microbiology*
  • Dental Caries Susceptibility
  • Genes, Bacterial*
  • Humans
  • Male
  • Molecular Sequence Data
  • Nucleic Acid Hybridization / methods*
  • Polymerase Chain Reaction / methods*
  • Sequence Analysis, DNA
  • Streptococcus mutans / genetics*
  • Streptococcus mutans / pathogenicity

Substances

  • DNA, Bacterial

Associated data

  • GENBANK/DQ072007
  • GENBANK/DQ072008