A versatile vector system for multiple gene expression in plants

Trends Plant Sci. 2005 Aug;10(8):357-61. doi: 10.1016/j.tplants.2005.06.001.

Abstract

Today, cloning vectors that have been specifically designed to facilitate the fusion, overexpression or down-regulation of a variety of genes in plant cells are available from various sources. In most cases, their basic design allows the cloning of a single target gene, typically under a specific promoter, in parallel with the expression of selection and/or marker genes from the same vector. However, new and versatile systems now exist that expand the user's choice to a large number of promoters and terminators, and various autofluorescent tags confer the ability to express multiple genes from a single transformation vector.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Cloning, Molecular / methods
  • Gene Expression Regulation, Plant*
  • Multigene Family
  • Plants / genetics*
  • Promoter Regions, Genetic
  • Terminator Regions, Genetic / genetics