Coral red fluorescence protein as genetic modified baculovirus tracer

J Biotechnol. 2005 Sep 29;119(3):255-9. doi: 10.1016/j.jbiotec.2005.04.019.

Abstract

Genetic modified baculovirus (GMBV) are among the most promising alternatives to chemical insecticides. One of the deterrents to the GMBV development is the lack of simple and cost-effective methods for monitoring their efficacy and ecology in fields. Here, we demonstrate the DsRed gene from coral can serve as a convenient GMBV tracer. Insect larvae, including Trichoplusia ni, Spodoptera exigua, and Spodoptera litura, infected the GMBV containing the DsRed gene can emit red fluorescence under sun light without any prosthetic apparatus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anthozoa
  • Baculoviridae / genetics*
  • Biotechnology / methods*
  • Fluorescence
  • Fluorescent Dyes / chemistry
  • Green Fluorescent Proteins / metabolism
  • Luminescent Proteins / chemistry*
  • Luminescent Proteins / genetics*
  • Microscopy, Fluorescence
  • Moths / virology
  • Red Fluorescent Protein
  • Spodoptera / virology

Substances

  • Fluorescent Dyes
  • Luminescent Proteins
  • Green Fluorescent Proteins