Abstract
Chitinolytic activity of Serratia marcescens Nima (130 U ml(-1)) was up to 43 times higher than those produced by other S. marcescens strains. This strain synthesized an endochitinase (Chi-60), an exochitinase (Chi-50) and a novel N-acetylglucosaminidase. This latter showed two putative isoforms (Chi-180.5 and Chi-180.8) with isoelectric points of 5 and 8.1, respectively.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylglucosaminidase / chemistry*
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Acetylglucosaminidase / genetics
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Bacillus thuringiensis / enzymology
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Biotechnology / methods*
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Chitinases / metabolism
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Electrophoresis, Gel, Two-Dimensional
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Glycoproteins / chemistry
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Glycoside Hydrolases / chemistry
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Hydrogen-Ion Concentration
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Isoelectric Focusing
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Protein Isoforms
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Serratia marcescens / enzymology*
Substances
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Glycoproteins
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Protein Isoforms
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Glycoside Hydrolases
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Chitinases
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Acetylglucosaminidase