Chitinases from Serratia marcescens Nima

Biotechnol Lett. 2005 May;27(9):649-53. doi: 10.1007/s10529-005-3661-1.

Abstract

Chitinolytic activity of Serratia marcescens Nima (130 U ml(-1)) was up to 43 times higher than those produced by other S. marcescens strains. This strain synthesized an endochitinase (Chi-60), an exochitinase (Chi-50) and a novel N-acetylglucosaminidase. This latter showed two putative isoforms (Chi-180.5 and Chi-180.8) with isoelectric points of 5 and 8.1, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylglucosaminidase / chemistry*
  • Acetylglucosaminidase / genetics
  • Bacillus thuringiensis / enzymology
  • Biotechnology / methods*
  • Chitinases / metabolism
  • Electrophoresis, Gel, Two-Dimensional
  • Glycoproteins / chemistry
  • Glycoside Hydrolases / chemistry
  • Hydrogen-Ion Concentration
  • Isoelectric Focusing
  • Protein Isoforms
  • Serratia marcescens / enzymology*

Substances

  • Glycoproteins
  • Protein Isoforms
  • Glycoside Hydrolases
  • Chitinases
  • Acetylglucosaminidase