Purification and determination of plant hormones auxin and abscisic acid using solid phase extraction and two-dimensional high performance liquid chromatography

P I Dobrev; L Havlícek; M Vagner; J Malbeck; M Kamínek

doi:10.1016/j.chroma.2005.02.091

Purification and determination of plant hormones auxin and abscisic acid using solid phase extraction and two-dimensional high performance liquid chromatography

J Chromatogr A. 2005 May 20;1075(1-2):159-66. doi: 10.1016/j.chroma.2005.02.091.

Authors

P I Dobrev¹, L Havlícek, M Vagner, J Malbeck, M Kamínek

Affiliation

¹ Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 135, 16502 Praha 6, Czech Republic. dobrev@ueb.cas.cz

PMID: 15974129
DOI: 10.1016/j.chroma.2005.02.091

Abstract

A method for separation and purification of plant hormones auxin and abscisic acid based on mixed mode reversed-phase anion-exchange solid phase extraction and two-dimensional HPLC was developed. Two-dimensional HPLC in "heart cutting" mode was very efficient in the purification of these two hormones. Its purification power is high enough to allow reliable on-line quantification of both hormones even with non-selective detectors.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Abscisic Acid / analysis
Abscisic Acid / isolation & purification*
Chromatography, High Pressure Liquid / methods*
Indoleacetic Acids / analysis
Indoleacetic Acids / isolation & purification*
Plant Growth Regulators / analysis
Plant Growth Regulators / isolation & purification*
Reproducibility of Results

Substances

Indoleacetic Acids
Plant Growth Regulators
Abscisic Acid