We assessed the effects of supplementation of broiler breeder diets with polyunsaturated fatty acids (PUFA) and organic Se on hatching egg quality during storage. Broiler breeders (n = 352) were fed 1 of 4 diets: SO (soybean oil with no added Se), SO with Se (SO+Se), fish oil (FO), or FO and Se (FO+Se). Eggs from prepeak (23 wk) and peak production (27 wk) breeders were stored for 14 d under typical (15 degrees C, 78% RH) conditions. Eggs were analyzed for albumen Haugh units (HU) and pH, shell characteristics, egg component weight, Se content, and fatty acid (FA) profile. The efficiency of transfer of Se into the egg was greater in low Se treatments compared with in high Se treatments. The majority of Se from the low Se treatments was deposited in the yolk, whereas in high Se treatments, the Se was deposited evenly in the yolk and albumen. In the low Se treatments, the Se content of yolk and albumen was lower than in the high Se treatments and decreased as age increased. Albumen HU decreased with increased storage, although high Se treatments had greater HU compared with low Se treatments. Eggs from the FO treatment were smaller with thinner eggshells compared with the SO treatment, but addition of Se to the FO diets ameliorated some of these effects. The egg yolk FA profile from the SO+Se treatment was altered compared with that from the SO treatment, perhaps due to interactions between dietary PUFA and Se-dependent enzyme systems. Hatching egg quality during storage was affected by dietary PUFA and Se content, as well as broiler breeder age.