Comparison of replicative senescence and stress-induced premature senescence combining differential display and low-density DNA arrays

Thierry Pascal; Florence Debacq-Chainiaux; Aline Chrétien; Coralie Bastin; Anne-France Dabée; Vincent Bertholet; José Remacle; Olivier Toussaint

doi:10.1016/j.febslet.2005.05.056

Comparison of replicative senescence and stress-induced premature senescence combining differential display and low-density DNA arrays

FEBS Lett. 2005 Jul 4;579(17):3651-9. doi: 10.1016/j.febslet.2005.05.056.

Authors

Thierry Pascal¹, Florence Debacq-Chainiaux, Aline Chrétien, Coralie Bastin, Anne-France Dabée, Vincent Bertholet, José Remacle, Olivier Toussaint

Affiliation

¹ Unit of Research on Cellular Biology, Department of Biology, University of Namur (FUNDP), Rue de Bruxelles, 61 B-5000 Namur, Belgium.

PMID: 15963989
DOI: 10.1016/j.febslet.2005.05.056

Abstract

Human diploid fibroblasts (HDFs) exposed to subcytotoxic stress display many features of senescence. Using differential display RT-PCR, gene expression of HDFs in premature senescence induced by tert-butylhydroperoxide or ethanol and in replicative senescence was compared to gene expression of HDFs at early cumulative population doublings. Thirty genes of known function were identified from the 265 differentially displayed cDNA fragments. A customized low-density array allowed to confirm the relative level of the corresponding 30 transcripts. We found differential expression of genes coding for proteins implicated namely in growth arrest (PTEN, IGFBP-3, LRP-1 and CAV1), senescent morphogenesis (TGF-beta1 and LOXL2) and iron metabolism (TFR and FTL).

Publication types

Comparative Study

MeSH terms

Cellular Senescence / genetics*
Ethanol / toxicity
Fibroblasts / drug effects
Fibroblasts / metabolism
Gene Expression Profiling
Gene Expression* / drug effects
Humans
Iron / metabolism
Lung / cytology
Oligonucleotide Array Sequence Analysis
tert-Butylhydroperoxide / toxicity

Substances

Ethanol
tert-Butylhydroperoxide
Iron