Abstract
Laminin alpha3 chain, a functionally key subunit of laminin-5, contains a large globular module (G module) which consists of a tandem repeat of five homologous LG modules (LG1-5). We previously demonstrated that the LG4 module of laminin alpha3 chain (alpha3 LG4) induces a matrix metalloproteinase-1 (MMP-1) expression through the interaction with syndecans leading to MAPK activation/IL-1beta expression signaling loop (Utani et al., J. Biol. Chem. 278, 34483-34490, 2003). Here, we show that a recombinant alpha3 LG4 and synthetic peptides containing syndecan binding motif induced a cell motility and a MMP-9 expression in ketarinocytes. The synthetic peptide (A3G756)-induced cell migration and MMP-9 upregulation were inhibited by each application of a heparin and an IL-1 receptor antagonist (IL-1RA), suggesting the involvement of syndecans and IL-1beta autocrine. Furthermore, the A3G756-induced cell motility was inhibited by an MMP-9 inhibitor and a neutralizing antibody of MMP-9, indicating induced cell motility was dependent on an MMP-9 activity. Taken these together, laminin-5 alpha3 LG4 module may play an important role in re-epithelialization at tissue remodeling.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Binding Sites / genetics
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Cell Movement / drug effects
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Cells, Cultured
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DNA, Complementary / genetics
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Gene Expression / drug effects
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Humans
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Interleukin 1 Receptor Antagonist Protein
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Keratinocytes / drug effects*
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Keratinocytes / physiology*
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Laminin / chemistry*
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Laminin / genetics
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Laminin / pharmacology*
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Matrix Metalloproteinase 9 / genetics
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Matrix Metalloproteinase 9 / metabolism*
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Membrane Glycoproteins / metabolism
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Peptide Fragments / chemistry
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Peptide Fragments / genetics
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Peptide Fragments / pharmacology
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Proteoglycans / metabolism
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Receptors, Interleukin-1 / antagonists & inhibitors
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / pharmacology
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Sialoglycoproteins / pharmacology
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Syndecans
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Tandem Repeat Sequences
Substances
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DNA, Complementary
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IL1RN protein, human
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Interleukin 1 Receptor Antagonist Protein
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Laminin
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Membrane Glycoproteins
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Peptide Fragments
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Proteoglycans
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RNA, Messenger
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Receptors, Interleukin-1
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Recombinant Proteins
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Sialoglycoproteins
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Syndecans
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laminin alpha 3
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Matrix Metalloproteinase 9