Abstract
We report a novel application for the operator-repressor titration (ORT) plasmid maintenance system. The ability of ORT to maintain a plasmid during production of DNA has been demonstrated previously. In this study, we have used the ORT system to maintain a plasmid during high cell density cultivation and expression of a recombinant protein. No evidence of plasmid loss was seen during protein expression at high cell densities. In addition, the quantity of protein produced using this system was similar to traditional plasmid maintenance systems.
Copyright 2005 Wiley Periodicals, Inc.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aldehyde-Lyases / analysis
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Aldehyde-Lyases / biosynthesis*
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Animals
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Escherichia coli / enzymology
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Escherichia coli / genetics*
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Fermentation
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Gene Expression Regulation, Bacterial / genetics
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Gene Expression Regulation, Bacterial / physiology*
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Industrial Microbiology / methods
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Operator Regions, Genetic*
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Plasmids* / genetics
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Plasmids* / metabolism
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Recombinant Proteins / analysis
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Recombinant Proteins / biosynthesis
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Repressor Proteins / genetics*
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Repressor Proteins / metabolism
Substances
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Recombinant Proteins
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Repressor Proteins
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Aldehyde-Lyases
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L-fuculosephosphate aldolase