Abstract
Genomic subtractive hybridization was used to design Prevotella nigrescens-specific primers and TaqMan probes. Based on this technique, a TaqMan real-time PCR assay was developed for quantifying four oral black-pigmented Prevotella species. The combination of real-time PCR and genomic subtractive hybridization is useful for preparing species-specific primer-probe sets for closely related species.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacteroidaceae Infections / microbiology
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Biofilms / growth & development*
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DNA Probes
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DNA, Bacterial / analysis
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Dental Plaque / microbiology
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Humans
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Mouth / microbiology*
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Nucleic Acid Hybridization / methods*
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Polymerase Chain Reaction / methods*
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Prevotella / classification
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Prevotella / genetics
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Prevotella / isolation & purification*
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Species Specificity
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Taq Polymerase
Substances
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DNA Probes
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DNA, Bacterial
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Taq Polymerase