The ability of an Actinomyces strain--Streptomyces spp. to produce extracellular proteases has been studied under varied cultivation conditions during the growth cycle. The activity of enzyme preparations precipitated from the culture liquid was determined with various substrates--gelatin, casein, fibrinogen, fibrin and collagen. The isolated enzyme complex possessed caseinolytic, fibrinolytic, thrombolytic and collagenolytic activities.