Abstract
Phosphatidylinositol-specific phospholipases (PI-PLCs) are virulence factors produced by many pathogenic bacteria, including Bacillus anthracis and Listeria monocytogenes. Bacillus PI-PLC differs from Listeria PI-PLC in that it has strong activity for cleaving GPI-anchored proteins. Treatment of murine DCs with Bacillus, but not Listeria, PI-PLC inhibited dendritic cell (DC) activation by TLR ligands. Infection of mice with Listeria expressing B. anthracis PI-PLC resulted in a reduced Ag-specific CD4 T cell response. These data indicate that B. anthracis PI-PLC down-modulates DC function and T cell responses, possibly by cleaving GPI-anchored proteins important for TLR-mediated DC activation.
Publication types
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Comparative Study
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Bacillus anthracis / enzymology*
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Bacillus anthracis / immunology*
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Bacillus anthracis / pathogenicity
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Bacillus cereus / enzymology
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Bacillus cereus / immunology
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Bacillus thuringiensis / enzymology
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Bacillus thuringiensis / immunology
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Cells, Cultured
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Dendritic Cells / immunology
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Dendritic Cells / metabolism
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Dendritic Cells / microbiology
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Down-Regulation / immunology*
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Glycosylphosphatidylinositols / antagonists & inhibitors
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Glycosylphosphatidylinositols / physiology
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Ligands
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Listeria monocytogenes / enzymology
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Listeria monocytogenes / immunology
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Lymphocyte Activation / immunology
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MAP Kinase Signaling System / immunology
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Membrane Glycoproteins / antagonists & inhibitors
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Membrane Glycoproteins / metabolism
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Mice
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Mice, Inbred C57BL
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Mitogen-Activated Protein Kinases / antagonists & inhibitors
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Mitogen-Activated Protein Kinases / physiology
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Phosphatidylinositol Diacylglycerol-Lyase / biosynthesis
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Phosphatidylinositol Diacylglycerol-Lyase / physiology*
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Phosphoinositide Phospholipase C
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Receptors, Cell Surface / antagonists & inhibitors
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Receptors, Cell Surface / metabolism
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T-Lymphocytes / immunology
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T-Lymphocytes / microbiology
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Toll-Like Receptors
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Virulence Factors / biosynthesis
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Virulence Factors / physiology*
Substances
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Glycosylphosphatidylinositols
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Ligands
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Membrane Glycoproteins
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Receptors, Cell Surface
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Toll-Like Receptors
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Virulence Factors
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Mitogen-Activated Protein Kinases
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Phosphoinositide Phospholipase C
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Phosphatidylinositol Diacylglycerol-Lyase