Magnetic resonance imaging at 9.4 T as a tool for studying neural anatomy in non-vertebrates

Catherine K Brinkley; Nancy H Kolodny; Susan J Kohler; David C Sandeman; Barbara S Beltz

doi:10.1016/j.jneumeth.2005.02.003

Magnetic resonance imaging at 9.4 T as a tool for studying neural anatomy in non-vertebrates

J Neurosci Methods. 2005 Jul 15;146(1):124-32. doi: 10.1016/j.jneumeth.2005.02.003. Epub 2005 Apr 7.

Authors

Catherine K Brinkley¹, Nancy H Kolodny, Susan J Kohler, David C Sandeman, Barbara S Beltz

Affiliation

¹ Department of Biological Sciences, 106 Central Street, Wellesley College, Wellesley, MA 02481, USA.

PMID: 15935229
DOI: 10.1016/j.jneumeth.2005.02.003

Abstract

This report describes magnetic resonance imaging (MRI) methods we have developed at 9.4 T for observing internal organs and the nervous system of an invertebrate organism, the crayfish, Cherax destructor. We have compared results acquired using two different pulse sequences, and have tested manganese (Mn(2+)) as an agent to enhance contrast of neural tissues in this organism. These techniques serve as a foundation for further development of functional MRI and neural tract-tracing methods in non-vertebrate systems.

Publication types

Comparative Study

MeSH terms

Animals
Astacoidea / anatomy & histology*
Astacoidea / physiology
Brain / anatomy & histology
Brain / physiology
Chlorides
Contrast Media
Ganglia, Invertebrate / anatomy & histology
Ganglia, Invertebrate / physiology
Magnetic Resonance Imaging / instrumentation
Magnetic Resonance Imaging / methods*
Magnetics
Manganese Compounds
Nervous System / anatomy & histology*
Neural Pathways / anatomy & histology
Neural Pathways / physiology
Neuroanatomy / instrumentation
Neuroanatomy / methods*

Substances

Chlorides
Contrast Media
Manganese Compounds
manganese chloride