Molecular cloning and expression of Ad4BP/SF-1 in the serial sex changing gobiid fish, Trimma okinawae

Yasuhisa Kobayashi; Tomoki Sunobe; Tohru Kobayashi; Masaru Nakamura; Norio Suzuki; Yoshitaka Nagahama

doi:10.1016/j.bbrc.2005.05.062

Molecular cloning and expression of Ad4BP/SF-1 in the serial sex changing gobiid fish, Trimma okinawae

Biochem Biophys Res Commun. 2005 Jul 15;332(4):1073-80. doi: 10.1016/j.bbrc.2005.05.062.

Authors

Yasuhisa Kobayashi¹, Tomoki Sunobe, Tohru Kobayashi, Masaru Nakamura, Norio Suzuki, Yoshitaka Nagahama

Affiliation

¹ Sesoko Station, Tropical Biosphere Research Center, University of the Ryukyus, 3422 Sesoko, Motobu-cho, Okinawa 905-0227, Japan. yasu-cob@amber.plala.or.jp

PMID: 15925324
DOI: 10.1016/j.bbrc.2005.05.062

Abstract

Involvement of Ad4BP/SF-1 was investigated using the serial sex changing goby Trimma okinawae. First, a cDNA encoding Ad4BP/SF-1 was cloned from ovarian follicles. The open reading frame of goby Ad4BP/SF-1 encodes a protein of 489 amino acids. Ad4BP/SF-1 was expressed in gonadal tissues, brain, and kidney. Second, transcript levels of Ad4BP/SF-1 were measured in the ovary and testis of the same individuals. Expressions were related to sexual phases. Moreover, ovarian expression of Ad4BP/SF-1 increased during vitellogenesis and declined sharply during the post-vitellogenic period. Finally, the expression profile of Ad4BP/SF-1 was measured during serial sex change in the ovary. Ad4BP/SF-1 increased in parallel with the onset of the female-phase and decreased as female became male. Therefore, Ad4BP/SF-1 probably acts as an important indirect regulator of oocyte growth and maturation at female-phase of serial sex changing gobiid fish T. okinawae.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
DNA, Complementary / metabolism
DNA-Binding Proteins / biosynthesis*
DNA-Binding Proteins / genetics*
Female
Fishes
Gene Expression Regulation*
Gonads / anatomy & histology*
Gonads / metabolism
Hermaphroditic Organisms*
Homeodomain Proteins
Male
Molecular Sequence Data
Open Reading Frames
Ovary / metabolism
RNA / metabolism
RNA, Messenger / metabolism
Receptors, Cytoplasmic and Nuclear
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, DNA
Sex Determination Processes*
Species Specificity
Steroidogenic Factor 1
Testis / metabolism
Tissue Distribution
Transcription Factors / biosynthesis*
Transcription Factors / genetics*
Transcription, Genetic

Substances

DNA, Complementary
DNA-Binding Proteins
Homeodomain Proteins
RNA, Messenger
Receptors, Cytoplasmic and Nuclear
Steroidogenic Factor 1
Transcription Factors
RNA