CpG motif in ATCGAT hexamer improves DNA-vaccine efficiency against lethal Pseudorabies virus infection in pigs

Vaccine. 2005 Aug 22;23(36):4532-40. doi: 10.1016/j.vaccine.2005.03.046.

Abstract

This study evaluated the adjuvant activity of a porcine-specific type A CpG-containing oligodeoxynucleotide (CpG-ODN) in a DNA-vaccine against Pseudorabies virus (PrV) infection. This vaccine, composed of three independent plasmids encoding PrV gB, gC and gD glycoproteins, was injected alone or was co-injected with CpG-ODN or control GpC-ODN. CpG-ODN improved the PrV-specific humoral immune response and provided better clinical protection against lethal PrV-infection. The PrV-specific cell-mediated immune response seemed also to be increased.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic / pharmacology*
  • Animals
  • Antibodies, Viral / blood
  • Body Weight
  • Immunization
  • Interferon-gamma / genetics
  • Interleukin-4 / genetics
  • Oligodeoxyribonucleotides / pharmacology*
  • Pseudorabies / prevention & control*
  • Pseudorabies Vaccines / immunology*
  • RNA, Messenger / analysis
  • Swine
  • Swine Diseases / prevention & control*
  • Vaccines, DNA / immunology*

Substances

  • Adjuvants, Immunologic
  • Antibodies, Viral
  • CPG-oligonucleotide
  • Oligodeoxyribonucleotides
  • Pseudorabies Vaccines
  • RNA, Messenger
  • Vaccines, DNA
  • Interleukin-4
  • Interferon-gamma