Abstract
This study evaluated the adjuvant activity of a porcine-specific type A CpG-containing oligodeoxynucleotide (CpG-ODN) in a DNA-vaccine against Pseudorabies virus (PrV) infection. This vaccine, composed of three independent plasmids encoding PrV gB, gC and gD glycoproteins, was injected alone or was co-injected with CpG-ODN or control GpC-ODN. CpG-ODN improved the PrV-specific humoral immune response and provided better clinical protection against lethal PrV-infection. The PrV-specific cell-mediated immune response seemed also to be increased.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adjuvants, Immunologic / pharmacology*
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Animals
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Antibodies, Viral / blood
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Body Weight
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Immunization
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Interferon-gamma / genetics
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Interleukin-4 / genetics
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Oligodeoxyribonucleotides / pharmacology*
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Pseudorabies / prevention & control*
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Pseudorabies Vaccines / immunology*
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RNA, Messenger / analysis
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Swine
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Swine Diseases / prevention & control*
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Vaccines, DNA / immunology*
Substances
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Adjuvants, Immunologic
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Antibodies, Viral
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CPG-oligonucleotide
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Oligodeoxyribonucleotides
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Pseudorabies Vaccines
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RNA, Messenger
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Vaccines, DNA
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Interleukin-4
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Interferon-gamma