Cell microarrays and RNA interference chip away at gene function

Douglas B Wheeler; Anne E Carpenter; David M Sabatini

doi:10.1038/ng1560

Cell microarrays and RNA interference chip away at gene function

Nat Genet. 2005 Jun:37 Suppl:S25-30. doi: 10.1038/ng1560.

Authors

Douglas B Wheeler¹, Anne E Carpenter, David M Sabatini

Affiliation

¹ Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Department of Biology, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA.

PMID: 15920526
DOI: 10.1038/ng1560

Abstract

The recent development of cell microarrays offers the potential to accelerate high-throughput functional genetic studies. The widespread use of RNA interference (RNAi) has prompted several groups to fabricate RNAi cell microarrays that make possible discrete, in-parallel transfection with thousands of RNAi reagents on a microarray slide. Though still a budding technology, RNAi cell microarrays promise to increase the efficiency, economy and ease of genome-wide RNAi screens in metazoan cells.

Publication types

Review

MeSH terms

Animals
Cells, Cultured
Gene Silencing
Genomics
Humans
Image Processing, Computer-Assisted
Oligonucleotide Array Sequence Analysis / methods*
Oligonucleotide Array Sequence Analysis / trends
RNA Interference*