tRNA actively shuttles between the nucleus and cytosol in yeast

Science. 2005 Jul 1;309(5731):140-2. doi: 10.1126/science.1113346. Epub 2005 May 19.

Abstract

Previous evidence suggested that transfer RNAs (tRNAs) cross the nuclear envelope to the cytosol only once after maturing in the nucleus. We now present evidence for nuclear import of tRNAs in yeast. Several export mutants accumulate mature tRNAs in the nucleus even in the absence of transcription. Import requires energy but not the Ran cycle. These results indicate that tRNAs shuttle between the nucleus and cytosol.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Active Transport, Cell Nucleus
  • Adenosine Triphosphate / metabolism
  • Cell Nucleus / genetics
  • Cell Nucleus / metabolism*
  • Cytosol / metabolism*
  • Introns
  • Mutation
  • RNA Precursors / metabolism
  • RNA, Fungal / metabolism*
  • RNA, Transfer / genetics
  • RNA, Transfer / metabolism*
  • RNA, Transfer, Amino Acid-Specific / metabolism*
  • RNA, Transfer, Amino Acyl / metabolism
  • RNA, Transfer, Ile / metabolism
  • RNA, Transfer, Pro / metabolism
  • RNA, Transfer, Tyr / metabolism
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / growth & development
  • Saccharomyces cerevisiae / metabolism*
  • Transcription, Genetic
  • ran GTP-Binding Protein / metabolism

Substances

  • RNA Precursors
  • RNA, Fungal
  • RNA, Transfer, Amino Acid-Specific
  • RNA, Transfer, Amino Acyl
  • RNA, Transfer, Ile
  • RNA, Transfer, Pro
  • RNA, Transfer, Tyr
  • Adenosine Triphosphate
  • RNA, Transfer
  • ran GTP-Binding Protein