To many Near Eastern archaeologists, the Late Bronze Age-Early Iron Age transition in the southern Levant indicates the emergence of a new ethnicity. The question remains, however, whether changes in the material culture are the result of an invasion of foreigners, or instead arose from shifting cultural and technical practices by indigenous peoples. This study utilized dental morphological traits to assess phenetic relationships between the Late Bronze Age site of Dothan (1500-1100 BC) and the Iron Age II site of Lachish (Tell ed-Duweir, 701 BC). Information on 30 dental crown and root traits was collected for 4,412 teeth, representing 392 individuals from Lachish and a minimum of 121 individuals from Dothan, using the Arizona State University Dental Anthropology System. Seventeen traits from Dothan and Lachish were compared with dentitions from a Byzantine Jerusalem monastery, Iron Age Italy, a Natufian group (early agrarians from the Levant), and a Middle Kingdom Egyptian site using C.A.B. Smith's mean measure of divergence statistic. The findings suggest that there are more similarities between Dothan and Lachish than either of them and other sites. This analysis indicates that the material culture changes were not the result of a foreign invasion. Rather, the Iron Age people of the southern Levant were related to their Bronze Age predecessors.