Enantioseparation of 2-O-beta-D-glucopyranosyl-2H-1,4-benzoxazin-3(4H)-one and its 7-chloro-derivative by capillary zone electrophoresis using native and substituted beta-cyclodextrins as chiral additives

Yujie Shi; Changhong Huo; Hongjun Yao; Rongfu Gao; Yuying Zhao; Bingjiu Xu

doi:10.1016/j.chroma.2005.03.027

Enantioseparation of 2-O-beta-D-glucopyranosyl-2H-1,4-benzoxazin-3(4H)-one and its 7-chloro-derivative by capillary zone electrophoresis using native and substituted beta-cyclodextrins as chiral additives

J Chromatogr A. 2005 Apr 29;1072(2):279-82. doi: 10.1016/j.chroma.2005.03.027.

Authors

Yujie Shi¹, Changhong Huo, Hongjun Yao, Rongfu Gao, Yuying Zhao, Bingjiu Xu

Affiliation

¹ School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100083, China.

PMID: 15887499
DOI: 10.1016/j.chroma.2005.03.027

Abstract

Efficient, rapid and inexpensive methods were established for the chiral separation of two glucopyranosyl compounds from plant extracts, by capillary zone electrophoresis (CZE). Baseline separation was achieved for both compounds. Several native cyclodextrins and their derivatives were tried as chiral selectors. CM-beta-CD and HP-beta-CD (with addition of acetonitrile in the buffer) gave rise to optimal chiral separation for the two compounds, respectively, each within a few minutes. The effects of several parameters on the chiral separation were studied.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Electrophoresis, Capillary / methods*
Stereoisomerism
beta-Cyclodextrins / chemistry*

Substances

beta-Cyclodextrins