One of the key techniques in the research of human motion analysis is the reconstruction of human spatial motion utilizing the evaluation of the anatomic points positions that can uniquely define the position of all anatomical segments. Upon the bases of direct linear transform and human biomechanical model, the method to reconstruct an image sequence shot by a stationary camera was described. The result of the reconstruction process was a set of data that is kinematically consistent with the biomechanical model used. A new method to calibrate camera was developed which can be completed in any world coordination system according to the practical situation. A practical motion was selected as the investigated subject. The predicting and experimental results were in good agreement. The benefits and shortcoming of the methodology were discussed.