Detection of canine distemper virus by reverse transcriptase-polymerase chain reaction in the urine of dogs with clinical signs of distemper encephalitis

T B Saito; A A Alfieri; S R Wosiacki; F J Negrão; H S A Morais; A F Alfieri

doi:10.1016/j.rvsc.2005.03.002

Detection of canine distemper virus by reverse transcriptase-polymerase chain reaction in the urine of dogs with clinical signs of distemper encephalitis

Res Vet Sci. 2006 Feb;80(1):116-9. doi: 10.1016/j.rvsc.2005.03.002.

Authors

T B Saito¹, A A Alfieri, S R Wosiacki, F J Negrão, H S A Morais, A F Alfieri

Affiliation

¹ Laboratório de Virologia Animal, Departamento de Medicina Veterinária Preventiva, Centro de Ciências Agrárias, Universidade Estadual de Londrina (UEL), Campus Universitário, Caixa Postal 6001, CEP: 86.051-990, Londrina, PR, Brazil.

PMID: 15878186
DOI: 10.1016/j.rvsc.2005.03.002

Abstract

In a prospective study we evaluated the use of the reverse transcriptase-polymerase chain reaction (RT-PCR) in urine samples to diagnose canine distemper virus in dogs with progressive neurological disease. A fragment of the nucleoprotein gene of canine distemper virus was amplified from the urine of 22 distemper dogs. The body fluids and leukocytes of 12 asymptomatic dogs were RT-PCR negative. RT-PCR of urine samples was more sensitive than serum and leukocytes and at least as sensitive as cerebrospinal fluid to screen for distemper in dogs with neurological signs and extraneural systemic signs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Distemper / diagnosis
Distemper / urine*
Distemper / virology*
Distemper Virus, Canine / genetics*
Distemper Virus, Canine / isolation & purification*
Dogs
Prospective Studies
Reverse Transcriptase Polymerase Chain Reaction / veterinary*