Traditional management of intersphincteric abscess is by incision and drainage involving division of fibres of the internal sphincter. Here, a less invasive method is described. Three patients with intersphincteric abscess identified by endoanal sonography were treated by ultrasound-guided needle aspiration under anaesthesia. None of the patients had complications or required postoperative wound care. All patients remained asymptomatic at a mean follow-up of 15.7 months (range, 12-18 months). Endoanal ultrasound-guided aspiration is a simple and safe method for the treatment of intersphincteric abscess.