Propolis is a kind of quality health foodstuff containing flavonoids, which is an important clinical effective component in many Chinese traditional medicines. The flavonoids could be used to reduce or clear free radicals, which manifests its medical functions. But there are no satisfactory methods to analyze the content of flavonoids in propolis. This paper reports a new method to determine the total effective value of flavonoids in propolis based on quenched effect for iodide-catalyzed electrochemiluminescence of luminol. It is a sensitive and simple method and might be a probable approach to examining the quality of propolis products.