Imaging magnetically labeled cells with magnetomotive optical coherence tomography

Opt Lett. 2005 Apr 1;30(7):747-9. doi: 10.1364/ol.30.000747.

Abstract

We introduce a novel contrast mechanism for optical coherence tomography (OCT) whereby the optical scattering of magnetically labeled cells is modified by means of an externally applied magnetic field. This modification is made through the addition of a small electromagnet to the imaging arm of a conventional OCT interferometer. We measure the magnetomotive OCT signal by differencing pairs of axial scans (A-scans) acquired with the magnetic field on and off. Magnetomotive contrast is demonstrated in bulk three-dimensional cell scaffolds containing macrophages labeled with microparticles of iron oxide, demonstrating magnetic-specific contrast over a dynamic range of 30 dB.

Publication types

  • Evaluation Study
  • Validation Study

MeSH terms

  • Animals
  • Cell Line
  • Contrast Media*
  • Ferrosoferric Oxide
  • Image Enhancement / methods*
  • Imaging, Three-Dimensional / methods*
  • Iron*
  • Macrophages / cytology*
  • Magnetics*
  • Mice
  • Mice, Inbred BALB C
  • Microspheres
  • Oxides*
  • Phantoms, Imaging
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Staining and Labeling / methods
  • Tomography, Optical Coherence / instrumentation
  • Tomography, Optical Coherence / methods*

Substances

  • Contrast Media
  • Oxides
  • Iron
  • Ferrosoferric Oxide