Rapid protocol for electroporation of Clostridium perfringens

Alena Jirásková; Libor Vítek; Johan Fevery; Tomás Ruml; Pavel Branny

doi:10.1016/j.mimet.2005.01.012

Rapid protocol for electroporation of Clostridium perfringens

J Microbiol Methods. 2005 Jul;62(1):125-7. doi: 10.1016/j.mimet.2005.01.012.

Authors

Alena Jirásková¹, Libor Vítek, Johan Fevery, Tomás Ruml, Pavel Branny

Affiliation

¹ Institute of Microbiology AS CR, Vídenská 1083, 14220 Prague 4, Czech Republic.

PMID: 15823401
DOI: 10.1016/j.mimet.2005.01.012

Abstract

A rapid and simple method has been developed for the electroporation of Clostridium perfringens with plasmid DNA. The new improvements, harvesting cells early in the logarithmic stage of growth, keeping the cells at room temperature and the absence of post-shock incubation on ice increased transformation efficiency by one order of magnitude.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Clostridium perfringens / genetics*
Electroporation / methods*
Plasmids / administration & dosage
Plasmids / genetics
Transformation, Bacterial / genetics*