Photothermal image flow cytometry in vivo

Opt Lett. 2005 Mar 15;30(6):628-30. doi: 10.1364/ol.30.000628.

Abstract

The capability of photothermal (PT) microscopy to image moving, unlabeled cells in real time in vivo is demonstrated in a study of circulating red and white blood cells in blood and lymph microvessels of rat mesentery. Potential applications of this optical tool, called PT flow cytometry, are discussed.

Publication types

  • Evaluation Study
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Blood Cells / cytology*
  • Flow Cytometry / instrumentation
  • Flow Cytometry / methods*
  • Image Interpretation, Computer-Assisted / methods
  • Mesentery / blood supply*
  • Mesentery / cytology*
  • Microcirculation / cytology*
  • Rats
  • Spectrum Analysis, Raman / instrumentation
  • Spectrum Analysis, Raman / methods*
  • Thermography / instrumentation
  • Thermography / methods*
  • Video Recording / instrumentation
  • Video Recording / methods