Chromatographic investigation of macromolecular affinity interactions

Galina A Platonova; Tatiana B Tennikova

doi:10.1016/j.chroma.2004.10.073

Chromatographic investigation of macromolecular affinity interactions

J Chromatogr A. 2005 Feb 11;1065(1):75-81. doi: 10.1016/j.chroma.2004.10.073.

Authors

Galina A Platonova¹, Tatiana B Tennikova

Affiliation

¹ Institute of Macromolecular Compounds, Russian Academy of Sciences, St Petersburg 199004, Russia.

PMID: 15782953
DOI: 10.1016/j.chroma.2004.10.073

Abstract

High-performance monolithic disk affinity chromatography was applied to the investigation of formation of complexes between (1) complementary polyriboadenylic and polyribouridylic acids, e.g. poly(A) and poly(U), respectively, (2) poly(A) and synthetic polycation poly(allylamine), pAA. Polyriboadenylic acid and poly(allylamine) were immobilized on macroporous disks (CIM disks). Quantitative parameters of affinity interactions between macromolecules were established using frontal analysis at different flow rates.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adsorption
Chromatography, Affinity / methods*
Chromatography, High Pressure Liquid / methods*
Kinetics
Poly A / chemistry*
Poly U / chemistry*

Substances

Poly A
Poly U