Enhanced sensitivity of surface plasmon resonance (SPR) immunoassays using a peroxidase-catalyzed precipitation reaction and its application to a protein microarray

J Immunol Methods. 2005 Feb;297(1-2):125-32. doi: 10.1016/j.jim.2004.12.007. Epub 2005 Jan 27.

Abstract

We describe a method to improve the sensitivity of surface plasmon resonance (SPR) immunoassays using a horseradish peroxidase (HRP)-catalyzed precipitation reaction. The precipitation reaction catalyzed by HRP bound to the SPR biosensor surface via a sandwich immunoassay induced a shift in the SPR angle. Human interferon (IFN)-gamma at concentrations ranging from 0.01 to 100 ng/ml was detectable by this method. We also show that this biocatalytic signal amplification method can be applied to SPR imaging (SPRI), in an immunoassay of multiple proteins on a protein microarray format.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Catalysis
  • Chemical Precipitation
  • Horseradish Peroxidase / chemistry*
  • Humans
  • Immunoenzyme Techniques*
  • Interferon-gamma / analysis
  • Protein Array Analysis / methods*
  • Sensitivity and Specificity
  • Surface Plasmon Resonance / methods*

Substances

  • Interferon-gamma
  • Horseradish Peroxidase