Effect of DNA structure on the formation of collagen-DNA complex

Michiko Kaya; Yoshiharu Toyama; Kenji Kubota; Yoshinobu Nodasaka; Masanori Ochiai; Motoyoshi Nomizu; Norio Nishi

doi:10.1016/j.ijbiomac.2004.11.005

Effect of DNA structure on the formation of collagen-DNA complex

Int J Biol Macromol. 2005 Mar;35(1-2):39-46. doi: 10.1016/j.ijbiomac.2004.11.005. Epub 2005 Jan 21.

Authors

Michiko Kaya¹, Yoshiharu Toyama, Kenji Kubota, Yoshinobu Nodasaka, Masanori Ochiai, Motoyoshi Nomizu, Norio Nishi

Affiliation

¹ Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo 060-0810, Japan.

PMID: 15769514
DOI: 10.1016/j.ijbiomac.2004.11.005

Abstract

Using various types of DNAs prepared from plasmid DNA, complete double-stranded DNA (ds.DNA) with linear and cyclic forms and double-stranded DNA coexisting with single-stranded DNA (ss.DNA), the structure and fibrillogenesis of the collagen-DNA complex were investigated by means of turbidity, transmission electron microscopy, and confocal laser-scanning microscopy. The rate of fibrillogenesis of the collagen-DNA complex significantly depends on the DNA structure. The structure of the fibrils formed in the complexes showed a marked difference between the ds.DNA and ss.DNA complexes with collagen. Spatial distribution of the DNA and collagen in the complexes suggests that the characteristic collagen-DNA interaction depends on the DNA forms.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biocompatible Materials / chemistry*
Collagen / chemistry*
DNA / chemistry*
DNA, Single-Stranded / chemistry*
Electrophoresis, Agar Gel
Microscopy, Confocal
Microscopy, Electron, Transmission
Nephelometry and Turbidimetry
Nucleic Acid Conformation*
Pepsin A / chemistry
Plasmids / metabolism
Protein Binding
Time Factors

Substances

Biocompatible Materials
DNA, Single-Stranded
atelocollagen
Collagen
DNA
Pepsin A