Abstract
We performed GeneChip analyses on RNA from Bacillus anthracis lethal toxin (LeTx)-treated RAW 264.7 murine macrophages to investigate global effects of anthrax toxin on host cell gene expression. Stringent analysis of data revealed that the expression of several mitogen-activated protein kinase kinase-regulatory genes was affected within 1.5 h post-exposure to LeTx. By 3.0 h, the expression of 103 genes was altered, including those involved in intracellular signaling, energy production, and protein metabolism.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antigens, Bacterial / pharmacology*
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Antigens, Bacterial / toxicity
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Bacillus anthracis / metabolism*
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Bacterial Toxins / pharmacology*
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Bacterial Toxins / toxicity
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Gene Expression Profiling
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Gene Expression Regulation*
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Macrophages / immunology
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Macrophages / metabolism*
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Mice
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Mitogen-Activated Protein Kinase Kinases / genetics
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Mitogen-Activated Protein Kinase Kinases / metabolism
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Oligonucleotide Array Sequence Analysis / methods*
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Proteins / genetics
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Proteins / metabolism*
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Transcription, Genetic
Substances
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Antigens, Bacterial
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Bacterial Toxins
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Proteins
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anthrax toxin
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Mitogen-Activated Protein Kinase Kinases