GeneChip analyses of global transcriptional responses of murine macrophages to the lethal toxin of Bacillus anthracis

Infect Immun. 2005 Mar;73(3):1879-85. doi: 10.1128/IAI.73.3.1879-1885.2005.

Abstract

We performed GeneChip analyses on RNA from Bacillus anthracis lethal toxin (LeTx)-treated RAW 264.7 murine macrophages to investigate global effects of anthrax toxin on host cell gene expression. Stringent analysis of data revealed that the expression of several mitogen-activated protein kinase kinase-regulatory genes was affected within 1.5 h post-exposure to LeTx. By 3.0 h, the expression of 103 genes was altered, including those involved in intracellular signaling, energy production, and protein metabolism.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens, Bacterial / pharmacology*
  • Antigens, Bacterial / toxicity
  • Bacillus anthracis / metabolism*
  • Bacterial Toxins / pharmacology*
  • Bacterial Toxins / toxicity
  • Gene Expression Profiling
  • Gene Expression Regulation*
  • Macrophages / immunology
  • Macrophages / metabolism*
  • Mice
  • Mitogen-Activated Protein Kinase Kinases / genetics
  • Mitogen-Activated Protein Kinase Kinases / metabolism
  • Oligonucleotide Array Sequence Analysis / methods*
  • Proteins / genetics
  • Proteins / metabolism*
  • Transcription, Genetic

Substances

  • Antigens, Bacterial
  • Bacterial Toxins
  • Proteins
  • anthrax toxin
  • Mitogen-Activated Protein Kinase Kinases