Abstract
Vitamin D3 upregulated protein 1 (VDUP1) is a stress-response gene that is upregulated by 1,25(OH)2D3 in tumor cells. The in vivo roles of VDUP1 were investigated by producing mice lacking VDUP1 (VDUP1-/- mice). VDUP1-/- mice showed minimal changes in the development of T and B cells, but there was a profound reduction in the numbers of natural killer (NK) cells. As well, these mice showed decreased NK activity. In the VDUP1-/- mice, the expression of CD122 was reduced, demonstrating that VDUP1 is required for CD122 expression and NK maturation. In addition, severe lymphoid hyperplasia in the small intestine was observed in VDUP1-/- mice. Taken together, these results suggest that VDUP1 is a critical factor for the development and function of NK cells in vivo.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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B-Lymphocytes / cytology
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B-Lymphocytes / immunology
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B-Lymphocytes / metabolism
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Carrier Proteins / genetics
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Carrier Proteins / immunology
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Carrier Proteins / metabolism*
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Cell Differentiation*
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Cell Line
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Gene Deletion
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Gene Expression Regulation
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Humans
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Hyperplasia / metabolism
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Hyperplasia / pathology
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Killer Cells, Natural / cytology
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Killer Cells, Natural / immunology*
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Killer Cells, Natural / metabolism*
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Mice
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Mice, Knockout
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Neoplasm Transplantation
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Neoplasms / immunology
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Neoplasms / metabolism
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Receptors, Interleukin-2 / metabolism
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T-Lymphocytes / cytology
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T-Lymphocytes / immunology
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T-Lymphocytes / metabolism
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Thioredoxins / genetics
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Thioredoxins / immunology
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Thioredoxins / metabolism*
Substances
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Carrier Proteins
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Receptors, Interleukin-2
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Txnip protein, mouse
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Thioredoxins