Abstract
Three new pyranonaphthoquinones: 5-hydroxy-6-methoxy-alpha-lapachone, 5,6-dihydroxy-a-lapachone and 4',5-dihydroxy-6-methoxy-alpha-lapachone, and two known compounds: lapachol and 5,5'-dihydroxy-3',4',7-trimethoxyflavanone, were isolated from the stem bark of Melloa quadrivalvis. Their structures were established by spectrometric data, mainly 1D- and 2D-NMR and mass spectra. The methylazoetetrazolium (MTT) method using viable cells of the strain Hep2 and the strain NCIH-292 demonstrated cytotoxic activity. The CI50 was also calculated. The chloroform extract and 5-hydroxy-6-methoxy-alpha-lapachone inhibited cell growth.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents, Phytogenic / isolation & purification*
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Antineoplastic Agents, Phytogenic / pharmacology
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Bignoniaceae / chemistry*
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Carcinoma, Hepatocellular / pathology
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Drug Screening Assays, Antitumor
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Liver Neoplasms / pathology
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Naphthoquinones / isolation & purification*
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Naphthoquinones / pharmacology
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Plant Bark / chemistry
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Plant Extracts / pharmacology
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Tumor Cells, Cultured
Substances
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4',5-dihydroxy-6-methoxy-alpha-lapachone
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5,6-dihydroxy-alpha-lapachone
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5-hydroxy-6-methoxy-alpha-lapachone
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Antineoplastic Agents, Phytogenic
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Naphthoquinones
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Plant Extracts
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lapachol