Abstract
Transcriptional repression of methylated genes can be mediated by the methyl-CpG binding protein MeCP2. Here we show that human Brahma (Brm), a catalytic component of the SWI/SNF-related chromatin-remodeling complex, associates with MeCP2 in vivo and is functionally linked with repression. We used a number of different molecular approaches and chromatin immunoprecipitation strategies to show a unique cooperation between Brm, BAF57 and MeCP2. We show that Brm and MeCP2 assembly on chromatin occurs on methylated genes in cancer and the gene FMR1 in fragile X syndrome. These experimental findings identify a new role for SWI/SNF in gene repression by MeCP2.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Cycle Proteins / physiology*
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Chromosomal Proteins, Non-Histone / physiology*
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DNA-Binding Proteins / physiology*
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Drosophila Proteins
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Fragile X Mental Retardation Protein
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Gene Silencing / physiology*
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Histones / physiology
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Humans
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Methyl-CpG-Binding Protein 2
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Mice
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Microscopy, Fluorescence
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Molecular Sequence Data
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NIH 3T3 Cells
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Nerve Tissue Proteins / genetics
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RNA-Binding Proteins / genetics
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Repressor Proteins / physiology*
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Trans-Activators / physiology*
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Transcription Factors / physiology*
Substances
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Cell Cycle Proteins
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Chromosomal Proteins, Non-Histone
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DNA-Binding Proteins
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Drosophila Proteins
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FMR1 protein, human
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Fmr1 protein, mouse
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Histones
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MECP2 protein, human
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Mecp2 protein, mouse
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Methyl-CpG-Binding Protein 2
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Nerve Tissue Proteins
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RNA-Binding Proteins
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Repressor Proteins
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SWI-SNF-B chromatin-remodeling complex
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Trans-Activators
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Transcription Factors
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brm protein, Drosophila
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Fragile X Mental Retardation Protein
Associated data
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RefSeq/NM_001379
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RefSeq/NM_003070
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RefSeq/NM_004992