Abstract
Transcription of the dinB gene, encoding DNA polymerase IV, is induced by the inhibition of cell wall synthesis at different levels. Using the beta-lactam antibiotic ceftazidime, a PBP3 inhibitor, as a model, we have shown that this induction is independent of the LexA/RecA regulatory system. Induction of dinB transcription mediated by ceftazidime produces an increase in the reversion of a +1 Lac frameshift mutation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphatases / physiology
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Bacterial Proteins / physiology
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Ceftazidime / pharmacology*
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Cell Wall / metabolism*
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DNA Helicases / physiology
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Enzyme Inhibitors / pharmacology*
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Escherichia coli / genetics*
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Escherichia coli / metabolism*
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Escherichia coli Proteins / genetics*
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Frameshift Mutation
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Gene Expression Regulation, Bacterial / drug effects*
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SOS Response, Genetics
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Serine Endopeptidases / physiology
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Transcription, Genetic
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beta-Galactosidase / analysis
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beta-Galactosidase / genetics
Substances
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Bacterial Proteins
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DinB protein, E coli
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Enzyme Inhibitors
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Escherichia coli Proteins
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LexA protein, Bacteria
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Ceftazidime
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beta-Galactosidase
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Serine Endopeptidases
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Adenosine Triphosphatases
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DNA Helicases