The fabrication of protein chip based on surface plasmon resonance for detection of pathogens

Byung-Keun Oh; Woochang Lee; Bum Suk Chun; Young Min Bae; Won Hong Lee; Jeong-Woo Choi

doi:10.1016/j.bios.2004.05.010

The fabrication of protein chip based on surface plasmon resonance for detection of pathogens

Biosens Bioelectron. 2005 Mar 15;20(9):1847-50. doi: 10.1016/j.bios.2004.05.010.

Authors

Byung-Keun Oh¹, Woochang Lee, Bum Suk Chun, Young Min Bae, Won Hong Lee, Jeong-Woo Choi

Affiliation

¹ Department of Chemical and Biomolecular Engineering, Sogang University, 1 Shinsu-dong, Mapo-gu, Seoul 121-742, Republic of Korea.

PMID: 15681203
DOI: 10.1016/j.bios.2004.05.010

Abstract

Protein chip based on surface plasmon resonance (SPR) was developed for detection of pathogens existing in contaminated environment, such as Escherichia coli O157:H7, Salmonella typhimurium, Legionella pneumophila, and Yersinia enterocolitica. Protein G was immobilized to endow the orientation of antibody molecules on the SPR surface. The pathogen binding of the protein chip was investigated by SPR spectroscopy. Consequently, it was found that the four kinds of pathogen could be selectively detected by using SPR-based protein chip.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Bacteria / immunology*
Bacteria / isolation & purification*
Biocompatible Materials / chemistry
Biosensing Techniques / instrumentation*
Biosensing Techniques / methods
Equipment Design
Equipment Failure Analysis
Immunoassay / instrumentation*
Immunoassay / methods
Materials Testing
Nerve Tissue Proteins / chemistry*
Nerve Tissue Proteins / immunology*
Protein Array Analysis / instrumentation*
Protein Array Analysis / methods
Surface Plasmon Resonance / instrumentation*
Surface Plasmon Resonance / methods

Substances

Biocompatible Materials
G-substrate
Nerve Tissue Proteins