Simple fibroblast-based assay to test the pyrazinamide susceptibility of Mycobacterium tuberculosis

Takemasa Takii; Sonomi Hamasaki; Kazue Hirano; Chiyoji Abe; Kikuo Onozaki

doi:10.1128/AAC.49.2.804-807.2005

Simple fibroblast-based assay to test the pyrazinamide susceptibility of Mycobacterium tuberculosis

Antimicrob Agents Chemother. 2005 Feb;49(2):804-7. doi: 10.1128/AAC.49.2.804-807.2005.

Authors

Takemasa Takii¹, Sonomi Hamasaki, Kazue Hirano, Chiyoji Abe, Kikuo Onozaki

Affiliation

¹ Department of Molecular Health Sciences, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-Dori, Mizuho-Ku, Nagoya 467-8603, Japan. ttakii@phar.nagoya-cu.ac.jp

Abstract

A simple fibroblast-based assay (SFA) was found to be efficient in evaluating the susceptibilities of clinical isolates of Mycobacterium tuberculosis to pyrazinamide (PZA). Forty-five clinical isolates were examined. The MICs of PZA for susceptible strains in an SFA were between 3.13 and 12.5 microg/ml, and the MICs of PZA for resistant strains were more than 100 microg/ml.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amidohydrolases / genetics
Antitubercular Agents / pharmacology*
Fibroblasts / microbiology*
Humans
Microbial Sensitivity Tests / methods*
Mycobacterium tuberculosis / drug effects*
Mycobacterium tuberculosis / genetics
Pyrazinamide / pharmacology*
Reverse Transcriptase Polymerase Chain Reaction
Tuberculosis / microbiology

Substances

Antitubercular Agents
Pyrazinamide
Amidohydrolases
PncA protein, Mycobacterium tuberculosis