Influence of immobilization parameters on growth and lactic acid production by Streptococcus thermophilus and Lactobacillus bulgaricus co-immobilized in calcium alginate gel beads

I Garbayo; C Vílchez; J M Vega; J E Nava-Saucedo; J N Barbotin

doi:10.1007/s10529-004-5134-3

Influence of immobilization parameters on growth and lactic acid production by Streptococcus thermophilus and Lactobacillus bulgaricus co-immobilized in calcium alginate gel beads

Biotechnol Lett. 2004 Dec;26(23):1825-7. doi: 10.1007/s10529-004-5134-3.

Authors

I Garbayo¹, C Vílchez, J M Vega, J E Nava-Saucedo, J N Barbotin

Affiliation

¹ Departamento de Química y CC.MM., (Area de Bioquímica), Facultad de Ciencias Experimentales, Universidad de Huelva, Campus del Carmen, 21017 Huelva, Spain. garbayo@uhu.es

PMID: 15672222
DOI: 10.1007/s10529-004-5134-3

Abstract

Streptococcus thermophilus and Lactobacillus bulgaricus were co-immobilized in different systems with varying calcium (0.1-1.5 M) and alginate (1-2%, w/v) concentrations. Highest lactic acid production was 35 g l(-1) when both bacteria were in high viscosity beads (1%, w/v alginate) hardened in 0.1 M CaCl2 . The gel bead composition affected size and distribution of entrapped lactic acid bacteria.

Publication types

Comparative Study

MeSH terms

Alginates / chemistry*
Biocompatible Materials / chemistry
Cell Proliferation
Cells, Immobilized / physiology
Coculture Techniques / methods*
Gels / chemistry*
Glucuronic Acid / chemistry*
Hexuronic Acids / chemistry*
Lactic Acid / biosynthesis*
Lactobacillus / physiology*
Materials Testing
Microspheres
Streptococcus thermophilus / physiology*
Viscosity

Substances

Alginates
Biocompatible Materials
Gels
Hexuronic Acids
Lactic Acid
Glucuronic Acid