A proteomic tool for protein identification from tandem mass spectral data

Proteomics. 2005 Mar;5(4):853-5. doi: 10.1002/pmic.200401033.

Abstract

Instead of using the probability mean, a simple and yet effective heuristic approach was employed to treat experimentally obtained tandem mass spectrometry (MS/MS) data for protein identification. The proposed approach is based on the total number (T) of identified experimental MS/MS data. To warrant the subsequent ranking, the total number of identified b- and y-type ions (Tb+y) must be greater than 50% of T. Peptides having the same T and Tb+y are either ranked by the contiguity of identified ions or discarded during identification. When compared to other protein identification tools, good agreement with the searched results was seen.

MeSH terms

  • Algorithms
  • Computational Biology / methods*
  • Databases, Protein
  • Internet
  • Mass Spectrometry / methods*
  • Peptides / chemistry
  • Proteins / chemistry
  • Proteomics / methods*
  • Software

Substances

  • Peptides
  • Proteins