Imaging protein behavior inside the living cell

Mol Cell Endocrinol. 2005 Jan 31;230(1-2):1-6. doi: 10.1016/j.mce.2004.10.011.

Abstract

The genetically encoded fluorescent proteins (FPs) have transformed studies in cell biology by allowing the behavior of proteins to be tracked within the natural environment of the living cell. Progressively more complex imaging methods are being used to measure the mobility, co-localization and interactions of proteins labeled with the FPs. This review provides an overview of recent developments in live-cell imaging techniques to analyze the subcellular distribution and interactions of proteins in living cells.

Publication types

  • Review

MeSH terms

  • Intracellular Space / chemistry
  • Luminescent Measurements
  • Luminescent Proteins / analysis*
  • Luminescent Proteins / chemistry*
  • Microscopy, Fluorescence
  • Proteins / analysis*
  • Spectrometry, Fluorescence

Substances

  • Luminescent Proteins
  • Proteins