Abstract
Dendritic cells (DC) are professional APC that have an extraordinary capacity to prime naive T cells. It has been reported that human DC subsets express distinct toll-like receptor (TLR), which influences their function. In mice, we observed that plasmocytoid DC (pDC) express a higher level of TLR9 compared with myeloid DC (mDC) cultured with GM-CSF. However, we demonstrated that stimulation with IFN-gamma is capable of upregulating TLR9 expression in mDC to a level comparable with expression in pDC. Consistent with this observation, IL-12 p40 and IL-6 mRNA expression and IL-12 p70 secretion in response to CpG-oligodeoxynucleotides are enhanced in mDC pretreated with IFN-gamma compared with untreated cells. Therefore, TLR-mediated responses of DC subsets may be influenced not only by signals delivered by pathogens but also by regulatory signals from cytokines such as IFN-gamma.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bone Marrow
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Cells, Cultured
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CpG Islands
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DNA, Bacterial / immunology
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DNA-Binding Proteins / drug effects
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DNA-Binding Proteins / genetics
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Dendritic Cells / drug effects
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Dendritic Cells / immunology*
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Female
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Immunity*
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Interferon-gamma / pharmacology*
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Interleukin-12 / genetics
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Interleukin-12 / metabolism
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Interleukin-12 Subunit p40
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Interleukin-6 / genetics
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Mice
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Mice, Inbred BALB C
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Oligodeoxyribonucleotides / immunology
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Oligodeoxyribonucleotides / pharmacology*
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Protein Subunits / genetics
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Protein Subunits / metabolism
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RNA, Messenger / analysis
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Receptors, Cell Surface / drug effects
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Receptors, Cell Surface / genetics
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Toll-Like Receptor 9
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Up-Regulation / drug effects
Substances
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DNA, Bacterial
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DNA-Binding Proteins
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Interleukin-12 Subunit p40
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Interleukin-6
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Oligodeoxyribonucleotides
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Protein Subunits
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RNA, Messenger
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Receptors, Cell Surface
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Tlr9 protein, mouse
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Toll-Like Receptor 9
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Interleukin-12
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Interferon-gamma