Three-dimensional structural analyses of human hair fibers and comparison of the different fibers were tried by using the Ultra-high Voltage Electron Microscope (UHVEM). The analysis condition, sample preparation, and a machine state were adjusted to the suitable condition for tilting observation of from -70 degree to +70 degree, at 2 degrees intervals. The tomography of hair fiber was successfully reconstructed from the different angle pictures with IMODE software in a computer. By using UHVEM, the various human hair fibers from Japanese and Caucasians were investigated and discussed about their structures.