[Comparison of denatured and non-denatured PAGE-silver-staining detection of PCR product of microsatellites]

Yi Chuan. 2004 Jul;26(4):522-4.
[Article in Chinese]

Abstract

The genomic DNAs from six chicken breeds in China were amplified using two microsatellite primers. The PCR products were detected by non-denatured and denatured PAGE gels respectively, and the gels were dyed by silver. There were distinct differences between the two kinds of gel. In non-denatured gels. There were many nonspecific bands while clear purposed bands were showed in denatured gels.

Publication types

  • Comparative Study
  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chickens / classification
  • Chickens / genetics*
  • DNA / genetics*
  • Electrophoresis, Polyacrylamide Gel / methods*
  • Microsatellite Repeats / genetics*
  • Polymerase Chain Reaction
  • Silver Staining

Substances

  • DNA