The polymorphism distributions of six STR loci, BM2113, BM1862, BMc701, BM2934, TGLA122,and BM720 were detected in cattle by Polymerase Chain Reaction and multiplex gel electrophoresis followed by silver staining. Gene frequency (P(i)),power of discrimination (DP), heterozygosity (H), polymorphism information content (PIC) and probability of paternity exclusion (PE) were calculated. All loci obey Hardy-Weinberg equilibrium. DP, H and PIC of BM2113 locus, and PE of TGLA122 locus are the biggest among six STR loci in Holstein Friesian. Cumulate DP of six STR loci is 0.99997, Cumulate PE of six STR loci is 0.98827. DP, H, PIC and PE of BM1862 loci are the biggest among six STR loci in beef. Cumulate DP of six STR loci is 0.99999, Cumulate PE of six STR loci is 0.99578. These results showed that the six STR loci could be used as linkage analysis, individual identification and paternity test in cattle.