Effect of inoculum size on detection of Candida growth by the BACTEC 9240 automated blood culture system using aerobic and anaerobic media

J Clin Microbiol. 2005 Jan;43(1):433-5. doi: 10.1128/JCM.43.1.433-435.2005.

Abstract

Simulated candidemia was produced with 20 Candida isolates at three inoculum sizes (100, 10, and 1 CFU/ml of blood). Growth detection was better with larger inocula. The time to growth detection was shorter with larger inocula. Inoculum size does effect Candida growth detection and time to detection in BACTEC 9240 automated systems.

Publication types

  • Evaluation Study
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Aerobiosis
  • Anaerobiosis
  • Blood / microbiology*
  • Candida / classification
  • Candida / growth & development*
  • Candida / isolation & purification
  • Candida albicans / growth & development
  • Candida albicans / isolation & purification
  • Candidiasis / microbiology
  • Culture Media
  • Fungemia / microbiology*
  • Humans
  • Microbiological Techniques
  • Reagent Kits, Diagnostic*

Substances

  • Culture Media
  • Reagent Kits, Diagnostic