Objective: To obtain recombinant human thrombopoietin (rhTpo).
Methods: rhTpo gene was cloned from fetal liver mRNA by means of RT-PCR and subcloned into permanent expressing plasmid RC/CMV in order to form a recombinant permanent expressing plasmid RC/CMV/Tpo.
Results and conclusion: After being transferred into CHO cells, the eukaryotic permanent expression of rhTpo, which can enhance murine megakaryocyte colony growth in vitro, was obtained.