Generation of clinical-grade monocyte-derived dendritic cells using the CliniMACS system

Thomas Putz; Hubert Gander; Reinhold Ramoner; Claudia Zelle-Rieser; Andrea Rahm; Walter Nussbaumer; Georg Bartsch; Lorenz Höltl; Martin Thurnher

doi:10.1385/1-59259-862-5:071

Generation of clinical-grade monocyte-derived dendritic cells using the CliniMACS system

Methods Mol Med. 2005:109:71-82. doi: 10.1385/1-59259-862-5:071.

Authors

Thomas Putz¹, Hubert Gander, Reinhold Ramoner, Claudia Zelle-Rieser, Andrea Rahm, Walter Nussbaumer, Georg Bartsch, Lorenz Höltl, Martin Thurnher

Affiliation

¹ Department of Urology, University of Innsbruck, Innsbruck, Austria.

PMID: 15585914
DOI: 10.1385/1-59259-862-5:071

Abstract

We describe the generation of monocyte-derived dendritic cells (MoDC) from leukapheresis products using the CliniMACS system from Miltenyi Biotec. In a clinical setting, the method turned out to be feasible for the generation of clinical-grade MoDC from patients with metastatic renal-cell carcinoma. MoDC generated with this system exhibited a fully mature phenotype as well as high migratory and T-cell stimulatory capacity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation
Cell Movement
Cell Separation / methods*
Dendritic Cells / cytology*
Dendritic Cells / immunology
Flow Cytometry
Humans
Immunomagnetic Separation / methods*
Leukapheresis
Lymphocyte Culture Test, Mixed
Monocytes / cytology*
Monocytes / immunology
Receptors, CCR7
Receptors, Chemokine / metabolism

Substances

CCR7 protein, human
Receptors, CCR7
Receptors, Chemokine