A comparative binding study of BIV Tat peptide against its TAR RNA duplex, RNA-DNA heteroduplex and DNA duplex

Bioorg Med Chem Lett. 2005 Jan 3;15(1):129-33. doi: 10.1016/j.bmcl.2004.10.030.

Abstract

Association between RNA and DNA strands to form RNA-DNA heteroduplex is important in many biological processes such as transcription, DNA replication and reverse transcription. Herein, binding affinities of a 17-mer BIV Tat peptide is compared with TAR DNA duplex, TAR RNA-DNA heteroduplex and TAR RNA duplex. It was observed that binding affinities of Tat peptide is comparable against DNA-RNA heteroduplex and RNA duplex, whereas DNA duplex binding is decidedly poor.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Circular Dichroism
  • DNA, Viral / chemistry
  • DNA, Viral / genetics
  • Gene Products, tat / chemistry
  • Gene Products, tat / genetics
  • Gene Products, tat / metabolism*
  • Immunodeficiency Virus, Bovine / genetics
  • Immunodeficiency Virus, Bovine / metabolism*
  • Nucleic Acid Heteroduplexes*
  • Protein Binding
  • RNA, Viral / chemistry
  • RNA, Viral / genetics

Substances

  • DNA, Viral
  • Gene Products, tat
  • Nucleic Acid Heteroduplexes
  • RNA, Viral