Abstract
Chick-quail chimeric studies were made to determine the origin of the cells of splenic ellipsoid. The ellipsoid is formed by supporting and phagocytic cells, which are embedded in a well-organized extracellular matrix. Splenic and bursal anlage of 6- to 6.5-day-old quail embryos were transplanted into the coelomic cavity of 3-day-old chick embryos and further incubated for 17 days. CD45+ chicken hemopoietic cells colonized both organs. They formed the cells of the ellipsoid and the periellipsoidal white pulp of the transplanted quail spleen. Chicken-specific collagen III was produced only in the donor quail spleen, but not in the bursa of Fabricius. The CD45+/collagen I+/collagen III+ cells are probably identical with the mammalian peripheral blood fibrocytes and contribute to the formation of supporting cells, whereas the CD45+/74.2+ ellipsoid-associated macrophages are of monocytic origin. We provide, for the first time, experimental evidence that peripheral blood fibrocytes exist in the avian species; they are present in the circulation of the chicken embryo and contribute to the organogenesis of the spleen.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens, Differentiation, B-Lymphocyte / biosynthesis
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Blood Cells / cytology*
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Bursa of Fabricius / cytology*
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Cell Differentiation
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Cell Transplantation
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Chick Embryo / cytology
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Chimera
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Collagen / metabolism
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Collagen Type I / biosynthesis
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Collagen Type III / biosynthesis
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Collagen Type III / metabolism
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Connective Tissue Cells / cytology
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Coturnix
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Embryonic Development*
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Extracellular Matrix / metabolism
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Gene Expression Regulation, Developmental*
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Hematopoiesis
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Hematopoietic Stem Cells / cytology
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Histocompatibility Antigens Class II / biosynthesis
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Immunohistochemistry
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Leukocyte Common Antigens / biosynthesis
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Macrophages / cytology
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Macrophages / metabolism
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Microscopy, Fluorescence
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Models, Anatomic
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Models, Biological
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Monocytes / cytology
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Phagocytosis
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Quail
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Recombinant Fusion Proteins / metabolism
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Spleen / cytology*
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Spleen / embryology*
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Time Factors
Substances
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Antigens, Differentiation, B-Lymphocyte
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Collagen Type I
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Collagen Type III
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Histocompatibility Antigens Class II
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Recombinant Fusion Proteins
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invariant chain
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Collagen
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Leukocyte Common Antigens