Binding properties of the conjugates of oligo(2'-O-methylribonucleotides) with minor groove binders targeted to double stranded DNA

D S Novopashina; A N Sinyakov; V A Ryabinin; A G Venyaminova; L Perrouault; E Brunet; C Giovannangeli; A S Boutorine

doi:10.1081/NCN-200026058

Binding properties of the conjugates of oligo(2'-O-methylribonucleotides) with minor groove binders targeted to double stranded DNA

Nucleosides Nucleotides Nucleic Acids. 2004 Oct;23(6-7):1015-22. doi: 10.1081/NCN-200026058.

Authors

D S Novopashina¹, A N Sinyakov, V A Ryabinin, A G Venyaminova, L Perrouault, E Brunet, C Giovannangeli, A S Boutorine

Affiliation

¹ Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russia. danov@niboch.nsc.ru

PMID: 15560096
DOI: 10.1081/NCN-200026058

Abstract

Design, synthesis, physico-chemical and in vitro biological studies of new pyrimidine oligo(2'-O-methylribonucleotide) conjugates with oligocarboxamide minor groove binders (MGB) and benzoindoloquinoline intercalator (BIQ) are described. These conjugates formed stable triple helices with the target double-stranded DNA and inhibited its in vitro transcription upon binding.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
DNA / chemistry
DNA / metabolism*
Ribonucleotides / metabolism*
Spectrophotometry, Ultraviolet

Substances

Ribonucleotides
DNA